Or MMP-9 and 514 bp for b-actin) and direct sequence analysis of the PCR product.Preparation of cell extracts and western blot analysisRBA-1 cells were made quiescent at confluence by incubation in serum-free DMEM/F-12 for 24 h. Growtharrested cells were incubated with LTA at 37 for the indicated times. When inhibitors were used, they were added 1 h prior to the application of LTA. Treatment of R