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Ca, MA) and rested for 24 hours. 0.1 and 1 M of TAK733 or parallel DMSO vehicle control were added in triplicate for 20 hours. Cells were incubated for 1 hour with 2.0 Ci with metabolic tracers chosen as analogues of PET tracers: 3H-DDG (American Radiolabeled Chemicals Inc., St. Louis, MO) in glucose-free RPMI 1640 (Invitrogen), or methyl-3H-thymidine (thymidine, Moravek Biochemicals Inc., Brea, C
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Ells can increase viral RNA production by over 100-fold making dendritic cells potent components in dengue pathogenesis[20]. Infected dendritic cells also contribute to vascular leak through the production of matrix metalloproteinases (MMPs). MMP-2, MMP-13, and MMP-9 were all dramatically increased in immature dendritic cells infected with DENV2. As a result cell-cell adhesion in cells co-cultured
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Ressed with the Roc1a promoter and regulatory regions, and the chimeric proteins contain an amino-terminal V5 tag. B, Control Roc1a and Roc1b or ANBR and BNAR constructs were transfected into S2 cells and cellular extracts were immunoprecipitated with anti-V5 antibodies and probed for the presence of Cul1 and Cul3 by immunoblotting. C, Control Roc1a and Roc2 or AN2R and 2NAR constructs were transf
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